Agronomic evaluations showed no changes in growth parameters, development, reproductive capacity and phenotypic characters between genetically modified cotton plants and the conventional cultivar. Genetically modified plants have been shown to be equally sensitive to biotic stress, so there is no evidence of change in their ability to survive and invasiveness. No direct effect on non-target pests and natural enemies was observed from the cultivation of genetically modified cotton events. No evidence of toxicity of Cry proteins was observed on selected non-target species after incorporation of purified proteins in the diet. Degradability analyzes of Cry proteins show that both reach undetectable values, although at different times, not posing a risk to microbiological and biochemical processes in the soil. Several parameters related to soil microbiota, such as protozoan population, mycorrhizal fungus, carbon and nitrogen biomass and soil respiration rate were not affected. The biodegradability of the roots and shoots of the TwinLink cotton plants was the same compared to the conventional strain. TwinLink cotton sheets showed greater degradability, which does not represent a change in their safety for the soil biota.